ChBE Seminar: Continuous Bioprocessing: New Challenges and Opportunities for Membrane Technology
Speaker: Andrew Zydney, Bayard D. Kunkle Chair & Professor of Chemical Engineering, Pennsylvania State University
Title: Continuous Bioprocessing: New Challenges and Opportunities for Membrane Technology
There is growing interest in the development of integrated continuous bioprocesses for the production of important biotherapeutics due to the potential for enhanced productivity, greater flexibility, and improved product quality. The transition from batch to continuous bioprocessing requires a major re-thinking of the downstream purification process, creating exciting new opportunities for membrane technology. This talk will focus on the use of continuous countercurrent staged membrane systems for initial product capture and for final formulation. Continuous Countercurrent Tangential Chromatography (CCTC) provides truly continuous steady-state product capture and purification using a column-free system in which the chromatographic operations are conducted on a flowing slurry using hollow fiber membranes to control the resin flow. Results using a Protein A affinity resin show high levels of host cell protein removal from a monoclonal antibody product, with the countercurrent staged providing significantly greater throughput with improved product yield and purification. A similar approach can be used for continuous selective precipitation of monoclonal antibodies, completely eliminating the high cost of the affinity resin. Countercurrent staged diafiltration has been developed as a new strategy for providing high levels of buffer exchange in a single-pass continuous format. These examples highlight how one can adapt membrane systems to continuous bioprocessing, providing exciting opportunities for improved manufacturing of high-value biopharmaceuticals.
Dr. Andrew L. Zydney is the Bayard D. Kunkle Chair and Professor of Chemical Engineering at The Pennsylvania State University. He also serves as Director of the Penn State site in the Membrane Science, Engineering, and Technology (MAST) Center. Professor Zydney's research is focused on the application of membranes in bioprocessing, including the purification of monoclonal antibodies, vaccines, and gene therapy agents. Dr. Zydney served as Editor-in-Chief of the Journal of Membrane Science from 2010-2019 and he is Past President of the North American Membrane Society (NAMS). He is the most recent recipient of the Alan S. Michaels Award for Innovation in Membrane Science and Technology, and he has previously received the American Chemical Society (ACS) Award in Separations Science and Technology, the Gerhold Award for Excellence in Separation Science, and the Excellence in Biological Engineering Publications award from the American Institute of Chemical Engineers (AIChE) among other recognitions. Professor Zydney has also received multiple teaching awards, including the Warren K. Lewis Award from AIChE, the Excellence in Teaching Award from the University of Delaware, and the Distinguished Teacher Award from the American Society for Engineering Education.